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  • Test Details
  • Lab Values
  • Clinical Significance
  • Treatment Options
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Explore this test

Test Code 625

Test Details

Vitamin D, 25-OH

25-hydroxy vitamin D is a hormone precursor that promotes bone, calcium and phosphorus metabolism.

Methodology

Antibody-based chemiluminescence

Patient Preparation

None

Preferred Specimen

Green Top Tube
(Lithium Heparin plasma separator tube with gel)

Alternate Specimen

Dried Blood collected on an AdvanceDx 100 card

Transport Temperature

Refrigerated (ship on frozen cold packs)

Stability

Refrigerated: 7 days

Lab Values

Lab Values

Vitamin D, 25-OH

  • Low: <30 ng/mL
  • Mid: 30–100 ng/mL
  • High: >100 ng/mL
Test Details
Clinical Significance

Clinical Significance

Vitamin D, 25-OH

Vitamin D insufficiency is associated with:1

  • Secondary hyperparathyroidism
  • Muscle weakness with increased fall risk
  • Myalgias

Vitamin D insufficiency may be associated with:2-4

  • Deleterious effects on cardiovascular system
  • Impaired pancreatic function
  • Increased mortality

References:

  1. Muscogiuri G, Sorcie GP, Ajjan R, et al. Can vitamin D deficiency cause diabetes and cardiovascular diseases? Present evidence and future perspectives. Nutr Metab Cardiovasc Dis. 2012;22(2):81-7.
  2. Goodman N, Cobin RH. Reproductive disorders. In: Camacho PM, Gharib H, Sizemore GW, eds. Evidence-Based Endocrinology. 3rd ed. Philadelphia, PA: Lippincott Williams & Wilkins. 2012:190-228.
  3. Castro MR, Gharib H. Thyroid disorders. In: Camacho PM, Gharib H, Sizemore GW, eds. Evidence-Based Endocrinology. 3rd ed. Philadelphia, PA: Lippincott Williams & Wilkins. 2012:43-80.
  4. Garber JR, Cobin RH, Gharib H, et al. Clinical practice guidelines for hypothyroidism in adults: cosponsored by the American Association of Clinical Endocrinologists and the American Thyroid Association. Endocr Pract. 2012;18(6):988-1028.
Lab Values
Treatment Options

Treatment Options

Vitamin D, 25-OH

  • Vitamin D, 25-OH – High levels indicated toxic levels of vitamin D. Consider discontinuing vitamin D supplements.
  • Vitamin D, 25-OH – Low vitamin D supplements. Vitamin D supplements can increase low blood levels of vitamin D, which have been associated with an increased risk of CVD and diabetes. Vitamin D supplements are frequently taken together with calcium to help maintain bone and muscle strength.
Clinical Significance